Expression of HCMV specific tmIgG.

<p><b>A)</b> Binding of the indicated IgG isotype mAbs to recombinant/purified HCMV gH protein as quantified by ELISA. <b>B)</b> Expression and gating of tmIgG and PDGFRa expression in transiently-transfected 293T cells <b>C)</b> Representative flow cytometry plots showing the frequency of HCMV SV40-GFP infected cells within the receptor-positive gate of tmIgG and PDGFRa transfected 293T cells 24hrs after virus inoculation. Cells infected at an MOI of 1 <b>D)</b> Quantification of HCMV SV40-GFP infected cells within the receptor-negative gate of tmIgG and PDGFRa in transfected 293T cells 24hrs after virus inoculation</p> <p>(PDF)</p>

Publication Details

Journal
Figshare
Published
2024-10-31
DOI
https://doi.org/10.1371/journal.ppat.1012683.s003
Primary Topic
Monoclonal and Polyclonal Antibodies Research
Type
article
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article

Expression of HCMV specific tmIgG.

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Monoclonal and Polyclonal Antibodies Research
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Expression of HCMV specific tmIgG.

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Abstract

<p><b>A)</b> Binding of the indicated IgG isotype mAbs to recombinant/purified HCMV gH protein as quantified by ELISA. <b>B)</b> Expression and gating of tmIgG and PDGFRa expression in transiently-transfected 293T cells <b>C)</b> Representative flow cytometry plots showing the frequency of HCMV SV40-GFP infected cells within the receptor-positive gate of tmIgG and PDGFRa transfected 293T cells 24hrs after virus inoculation. Cells infected at an MOI of 1 <b>D)</b> Quantification of HCMV SV40-GFP infected cells within the receptor-negative gate of tmIgG and PDGFRa in transfected 293T cells 24hrs after virus inoculation</p> <p>(PDF)</p>

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Monoclonal and Polyclonal Antibodies Research
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Expression of HCMV specific tmIgG. · Figshare (2024) | TGRS Research Map | TGRS