PCR detection of Foc TR4 using specific primers sets 13712F/13712R (this study), Foc TR4F/Foc TR4R [7], W2987F/W2987R [10], SIX1a-266-F/SIX1a-266-R [9], and VCG01213 16F/VCG01213 16R [8].

<p>Lane M, marker; lane 1, Foc TR4; lane 2, Foc race 1; lane 3, Foc race 2; lane 4, MAFF103008; lane 5, MAFF235154; lane 6, MAFF240805; lane 7, MAFF237022; lane 8, MAFF243476; lane 9, MAFF727508; lane 10, MAFF306716; lane 11, MAFF744004; lane 12, MAFF305544; lane 13, MAFF240804; lane 14, MAFF243255; lane 15, MAAFF241054; lane 16, MAFF306313; lane 17, MAFF305937; lane 18, MAFF103036; lane 19, MAFF235105; lane 20, MAFF744088; lane 21, MAFF103054; lane 22, MAFF305606; lane 23, MAFF240327; lane 24, MAFF103059; lane 25, MAFF103051; lane 26, MAFF240102; lane 27, MAFF305543; lane 28, MAFF305608; lane C, ddH2O.</p> <p>(PDF)</p>