Sampling locations for surface water collections of Chondria tumulosa environmental DNA (eDNA).

Sites were spread across Papahānaumokuākea Marine National Monument (PMNM) and Oʻahu. Biological replicates (water samples, n = 160) varied due to logistical constraints on expeditions. The number of triplicate qPCR positive detections per sample at each site are listed. The posterior probability (and 95% credible intervals, CI) of eDNA presence, occupancy (ψ), sample capture given site presence (θ₁₁), and detection probability given eDNA presence in a sample (p₁₁) were estimated using an eDNA model incorporating false-positives and augmented visual survey data. (DOCX)