References for defense gene IDs.

<div><p><i>Pratylenchus neglectus</i> and <i>P</i>. <i>thornei</i> are among the most destructive root lesion nematodes of wheat in the Pacific Northwest, United States of America and throughout the world. The aim of this study was to determine whether both nematode species were similar in their ability to induce defense genes in roots of wheat genotype Scarlet, and whether a combination of both species induced a different pattern of gene induction than each species alone. The long-term aspect of the research was to identify nematode-inducible promoters for deploying defense genes in roots in breeding programs. The root transcriptomes of genotype Scarlet were obtained after a one-week infection period with each nematode species separately, or both species combined. Root defense gene expression was induced for all three treatments relative to the no-nematode control, but <i>P</i>. <i>thornei</i> affected expression to a greater extent compared to <i>P</i>. <i>neglectus</i>. The species combination induced the highest number of defense genes. This result was not predicted from nematode enumeration studies, in which <i>P</i>. <i>thornei</i> colonization was substantially lower than that of <i>P</i>. <i>neglectus</i>, and the nematode combination did not show a significant difference. Quantitative real time polymerase chain reaction (qRT-PCR) assays for <i>Dehydrin2</i>, <i>Glucan endo-1</i>,<i>3-beta-glucosidase</i>, <i>1-cys-Peroxiredoxin</i>, <i>Pathogenesis-related protein 1</i> and <i>Late embryogenesis-abundant proteins 76</i> and <i>group 3</i> authenticated the induction observed in the transcriptome data. In addition, a near-isogenic line of Scarlet harboring genetic resistance to fungal soilborne pathogens, called Scarlet-Rz1, showed similar or higher levels of defense gene expression compared to fungus-susceptible Scarlet in qRT-PCR assays. Finally, transcriptome expression patterns revealed nematode-inducible promoters that are responsive to both <i>P</i>. <i>neglectus</i> and <i>P</i>. <i>thornei</i>.</p></div>