Phylogenetic tree of 1097 Ku70 protein sequences retrieved from OrthoDB.

Ku70 sequences were trimmed to obtain Ku70/80 beta-barrel sequences. 1097 sequences obtained were inferred by using the Maximum Likelihood method. Phylogenetic trees were drawn using PhyML. Automatic model selection based on the lowest BIC (Bayesian Information Criterion) was done using Smart Model Selection (SMS) in PhyML. Support for each branch was established using Shimodaira–Hasegawa [SH]-aLRT (approximate Likelihood Ratio Test). Nodes with support values of less than 50% were condensed using Mega-11 and the tree was annotated using FigTree and Abode Illustrator 2023. (PDF)