PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg2+.

PIP2 beads were incubated in nuclear lysates for 1 h at 4°C, washed, and subjected to WB detection of BRD4 protein. WB signals for each pull-down condition in each replicate were normalized to the highest signal (25 mM Mg2+). Statistical analysis was performed by Student’s t-test (n = 3). Error bars correspond to SEM. (PDF)

Publication Details

Journal: Figshare
Published: 2024-12-02
DOI: https://doi.org/10.1371/journal.pgen.1011462.s019
Primary Topic: Biotin and Related Studies
Type: article
Field-Weighted Citation Impact: 0.00

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article

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg²⁺.

Dec 2, 2024

Figshare

Biotin and Related Studies

article

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg²⁺.

December 2, 2024article en

Abstract

Figshare

Openalex Percentile: Top 72%

Biotin and Related Studies

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PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg2+. · Figshare (2024) | TGRS Research Map | TGRS

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg&lt;sup&gt;2+&lt;/sup&gt;.

Publication Details

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg2+.

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg2+.

Abstract

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg<sup>2+</sup>.

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg²⁺.

PIP2-conjugated agarose beads pull-down assays from nuclear lysates with the addition of 30 μg nuclear RNA extract at increasing concentrations of Mg²⁺.