Expressing <i>Δ-Clk</i> in the LN<sub>v</sub>s results in morphology and behavioral phenotypes.

<p>(A) Representative timeline. Flies were raised in LD at 28°C for their entire lifespan. Behavioral assays and dissections were performed within days 6–8 post-eclosion at ZT2-3. Experiments were conducted at 28°C. (B) Fisher’s tests were used to compare the percent of rhythmic flies of each indicated genotype (additional quantifications can be found in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011441#pgen.1011441.t001" target="_blank">Table 1</a>). ** P < 0.01, *** P < 0.001. For each genotype: 21 ≤ n ≤ 26. (C-D) Additional quantifications of effects of <i>Clk</i><sup><i>RNAi</i></sup> expression in the <i>Pdf</i>+ cells in adult brains. Neither the sLN<sub>v</sub> total projection length (C) nor the lLN<sub>v</sub> projections (D) were affected. Datasets were quantified with ordinary one-way ANOVA tests followed by Tukey’s Multiple Comparisons tests. For each genotype: 16 ≤ n ≤ 22. (E) Fisher’s tests were used to compare the percent of rhythmic flies of each indicated genotype (additional quantifications shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011441#pgen.1011441.t001" target="_blank">Table 1</a>). For each genotype: 27 ≤ n ≤ 31. (F-G) Effects of <i>Δ-Clk</i> expression in the <i>Pdf</i>+ cells in adult brains. The sLN<sub>v</sub> total projection length (F) and the lLN<sub>v</sub> projections (G) were quantified using one-way ANOVA tests followed by Tukey’s Multiple Comparisons tests. Each dot corresponds to one brain. For each genotype: 17 ≤ n ≤ 22. (H-J) Mann-Whitney tests were used to compare nuclear PER intensity levels in the sLN<sub>v</sub>s (H), lLN<sub>v</sub>s (J), and LN<sub>d</sub>s (J) in flies of the indicated genotypes. Flies were raised at constant 28°C for their entire lifespan and dissections were performed at ZT2-3 * p < 0.05, ** P < 0.01, *** P < 0.001. Error bars indicate SEM.</p> <p>(PDF)</p>