Splicing changes at or adjacent to different classes of introns in RBP-knockdown versus control datasets.

<p>Splicing changes for (A) FLEXIs, (B) other short introns, or (C) long introns in ENCODE knockdown datasets for Hep G2 and K-562 cells were calculated using rMATS (<a href="https://rnaseq-mats.sourceforge.io/" target="_blank">https://rnaseq-mats.sourceforge.io</a>). The Figure shows Empirical Cumulative Distribution Function (ECDF) plots for inclusion of retained introns (RI) or skipped exons (SE) adjacent to introns that have (red) or do not have (gray) a CLIP-seq-identified binding site for the indicated RBP in ENCODE knockdown datasets. Red curves shifted to the right or left of the control (gray) indicate an increase or decrease, respectively, in retained introns and skipped exons as indicated at the top of each set of plots. Statistical significance was calculated by Kolmogorov-Smirnov test. Plots are shown only from those RBPs whose knockdown resulted in a significant change (p≤0.05). Names of RBPs are color coded by protein function as indicated in the Figure. Blank spaces were left for datasets that were not available for an RBP in one of the two cell lines. Axes labels are shown in the key at the bottom right.</p> <p>(PDF)</p>