Differentiation of intestinal cell types are not affected by Notch dimerization deficiency.

<p>(A) Fold change of <i>Lgr5</i>, <i>Ascl2</i>, <i>Prom1</i> gene expression in jejunum relative to wild type mice analyzed by qRT- PCR. n = 3 mice per group. Quantitative data are presented as mean ± SEM. ns-Not Significant (B-C) Immunofluorescence staining of Sox9 of jejunum (B) and colon (C). (D) Jejunum immunofluorescence staining of Cleaved Caspase-3. (E) Representative images of MMP7 (Paneth cell marker) and PCNA immunofluorescence in jejunum. (F-H) Representative jejunum immunofluorescence images of CgA (enteroendocrine cells, F), Dcamkl1 (tuft cells, G), and Muc2 (secretory cells, H). Scale bars = 100 μm.</p> <p>(PDF)</p>