Fluorescence recovery after photobleaching of SNB-1::SpH in cholinergic motor neurons.

<p>(A) Representative images of pre-bleach(a), post-bleach(b), and post-recovery(c) of SNB-1::SpH labeled vesicles in the dorsal nerve cords in wild type animals expressing SNB-1::SEP in cholinergic motor neurons (<i>Punc-17</i>). Yellow circle marks the ROI of a single SpH punctum. (B) Plot profile of the indicated ROI is shown, indicating the points of measurements of pre-bleach, post-bleach and post-recovery used in calculating % recovery (described in <i>Materials and Methods</i>).</p> <p>(PDF)</p>