LC-MS analysis of T4 DNA composition.

<p>(<b>A-B</b>) Extracted ion chromatogram overlay of nucleotide mass traces in T4 WT DNA (front) and NgTET-treated T4 DNA (back). Data exemplifies differences in relative abundances of cysteine nucleotides (dC, 5hmdC, 5fdC, 5cadC) (<b>B</b>) while dG, dT and dA (<b>A</b>) show similar trends. Signal intensities have been normalized against the overall nucleotide signal in the respective sample to correct for differences in sample concentration and injection volume. (<b>C-D</b>) Extracted ion chromatogram of presumed 5ghmdC (<b>C</b>). The fragmentation pattern of presumed 5ghmdC corresponds to the predicted one (<b>D</b>). (<b>E</b>) Relative abundance of cytosine derivatives in T4 phage isolated from different strains (two-sided Student´s t-test, *—<i>P</i><sub><i>signif</i></sub> < 0.05, **—<i>P</i><sub><i>signif</i></sub> < 0.025; n.d.: not detected). <i>n</i> = 3 biological replicates.</p> <p>(PDF)</p>