S21 Fig -

<p>Manipulation of PIP2 level by PIP5KA and SHIP2 knock-down (relevant to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011462#pgen.1011462.g005" target="_blank">Fig 5C and 5D</a>). <b>(A)</b> Microscopy confirmation of the manipulation of PIP2 levels induced by depletion of PIP5KA and SHIP2 enzymes. Statistical analysis was performed using Student’s t-tests (**** P < 0.0001), n = 3, n = 4, N = 37 KD control cells, KD PIP5KA N = 44, and KD SHIP2 N = 50 cells, respectively). Error bars correspond to SEM. <b>(B)</b> WB analysis of the efficacy of PIP5KA and SHIP2 siRNA depletion and its effect on BRD4 protein levels.</p> <p>(PDF)</p>