Related to Fig 2.

<p>(A) The PZ mitotic index in animals bearing the mutant alleles or allele combinations indicated. <i>daf-18(nr2037)</i>, <i>akt-1(mg144)</i> and <i>daf-16(mu86)</i> rescue the lower mitotic index seen in <i>daf-2(e1370)</i> animals. Dots represent one PZ and one PZ was assessed per animal. (B) The DAF-16::GFP nuclear-to-cytoplasmic ratio (N:C) is elevated in GSPCs from animals in which <i>daf-2</i> was knocked down by RNAi. Small dots represent individual GSPCs, larger dots represent the mean value per gonad arm/PZ. (C) Single time point maximum intensity projections showing DAF-16::GFP nuclear localization over the course of a GSPC mitosis. Numbers indicate time in minutes relative to mitosis start, as inferred from HIS-11::mCH variance. Scale bar = 5 μm. For (A) and (B), boxplots show the median, interquartile range and most extreme values not considered statistical outliers. n.s. = <i>p</i> > 0.05, * = <i>p</i> < 0.05, *** = <i>p</i> < 0.001. Summary statistics and statistical tests used for all figure panels are given in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011351#pgen.1011351.s010" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>