SDS-PAGE analysis of the CLP-containing peaks in the RP-HPLC profiles of B. arietans venoms.

<p>Fractions from the HPLC traces shown in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0012570#pntd.0012570.g002" target="_blank">Fig 2</a> in the main text were subject to SDS-PAGE under reducing conditions and the those containing a pair of bands in the 13–18 kDa region typical of alpha and beta CLP subunits are shown [left hand gels]. These same fractions were also subjected to SDS-PAGE under non-reducing conditions [right hand gels] and most contained a strong band at 28–29 kDa corresponding to the intact heterodimeric CLP. In some minor forms [TZA 3, NGA 2 and SA 4] a 70–75 kDa protein was observed under non-reducing conditions and these are likely to be high molecular weight forms of CLPs. The SVMP peaks were identified by co-elution of the respective SVMPIIs purified in a separate study [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0012570#pntd.0012570.ref052" target="_blank">52</a>]. The gels used were BioRad 4–20% acrylamide, stained with Coomassie Blue R250.</p> <p>(DOCX)</p>