Using limms to identify statistically significant changes in a complex comparison reveals metabolites with unexpected patterns of change.

<p>Volcano plots of metabolites altered by disruption of function using a surrogate genetic system expressing the human vitamin B6-dependent enzyme cystathionine beta-synthase (CBS) in <i>S</i>. <i>cerevisiae</i>. Contrasts of the CBS major allele (MA) grown with high (400 ng/ml) or low (1 ng/ml) vitamin B6, compared to the G307S mutation at high (400 ng/ml) or low (1 ng/ml) vitamin B6, or under methionine replete versus starvation conditions. Annotations based on isotopically labeled, pooled standards are shown and tracked across conditions that affected the CBS cofactor, enzyme function, or substrate availability. Data for S3 Fig are provided in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002813#pbio.3002813.s013" target="_blank">S1 Data</a>.</p> <p>(PDF)</p>