Determination of structural identity by co-elution of the synthetic compounds with the natural Tb molecules.

<p>(A–C) The unknown, <i>Rv3378c</i>-dependent masses consistent with tuberculosinylguanosine and tuberculosinylinosine do not match synthetic tuberculosinylguanosine and tuberculosinylinosine. (A) Chemical structure of 1-tuberculosinylguanosine and overlayed extracted ion chromatograms of whole cell lipids from Mtb H37Ra and synthetic 1-tuberculosinylguanosine measured in the positive mode. (B) Chemical structure of 1-tuberculosinylinosine and overlayed extracted ion chromatograms of whole cell lipids from Mtb H37Ra, synthetic 1-tuberculosinylinosine and synthetic 1-tuberculosinylinosine spiked into H37Ra extract, measured using positive (B) and negative (C) mode mass spectrometry.</p> <p>(PDF)</p>