Ancestral state reconstruction of cacti association across the Saccharomycotina.

<p>The number of independent events of cacti association were inferred by performing an ancestral state reconstruction using the MBASR toolkit [<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002832#pbio.3002832.ref135" target="_blank">135</a>] and the topology of the species tree presented in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002832#pbio.3002832.g001" target="_blank">Fig 1</a>. State 0 (red) means absence of the trait while State 1 (orange) means presence of the trait and were attributed according to <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002832#pbio.3002832.s008" target="_blank">S1 Table</a> (considering presence of the trait only the strictly cactophilic species). For each node in the tree, probability of the trait being present (orange) or absent (red) is represented as a pie chart. In each terminal branch, red or orange circles represent the extant state (used as an input) for each species. Numbers next to each node represent the node identification.</p> <p>(PDF)</p>