Gene expression of the <i>nap</i> island in <i>N</i>. <i>gonorrhoeae</i>.

(A) RT-qPCR on all genes of the <i>nap</i> locus of strain FA1090. Samples were recovered from GW liquid cultures in flasks at different time points, and gene expression was normalised based on samples recovered from plates and resuspended in GW (T0). Error bars represent standard deviation from the mean (<i>n</i> = 3–4). One-way ANOVA was performed on data with minimum 2-fold change (dotted lines) compared to T0 (<i>p</i> < 0.033, *; <i>p</i> < 0.002, **; <i>p</i> < 0.001, ***). Growth curves were performed in parallel (right bottom panel). The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003001#pbio.3003001.s020" target="_blank">S8 Data</a>. (B) Predicted ribosome binding sites (RBS) and promoter regions (grey arrows) in the <i>nap</i> locus of strain FA1090. RBS were manually annotated based on the presence of a GGA enriched sequence −3 to −10 of an ATG start codon; promoter regions predicted by BPROM (<a href="http://softberry.com/" target="_blank">http://softberry.com</a>). (C, D) Pink arrows represent repeats of Correia elements (CE), while blue boxes show integration host factor (IHF) binding sites, as defined previously (<a href="https://doi.org/10.1016/s0378-1119(01)00725-9" target="_blank">10.1016/s0378-1119(01)00725-9</a> and <a href="https://doi.org/10.1016/s0014-5793(02)02882-x)" target="_blank">10.1016/s0014-5793(02)02882-x)</a>, respectively. Annotations were performed with Snap Gene. (PDF)