Ddt (Mif-like) and Hsp70 are not localized to the surfaces of esAEVs.

<p><b>(A)</b> A representative image of an esAEV administered a control stain of Secondary Alone (Scale bar = 200 nm), Hsp70 staining (Scale bar = 200 nm), Ddt staining (Scale bar = 400 nm), Annexin staining (Scale bar = 200 nm). (B) Quantitative representation of nanogold particles for Secondary Alone (mean+/−SEM = 0.000 +/− 0.000), Ddt (mean = 4.350 +/− 0.8438), Annexin V (mean = 41.64 +/− 5.524), and Hsp70 (13.19 +/− 3.370). Each dot represents an individual esAEV as represented in panels A. <i>n</i> = 27, Secondary Alone. <i>n</i> = 39, Annexin V. <i>n</i> = 20, Ddt. <i>n</i> = 16, Hsp70. The difference between means for Annexin V and Ddt was significant (adjusted <i>p</i>-value <0.0001). There was not a statistically significant difference in means between Ddt, Hsp70, and Secondary alone (<i>p</i> = 0.6939). (C) Representative images of Hsp70 antibody stains in zebrafish larvae. (D) The fluorescent intensity of Hsp70 antibody after MTZ treatment. <i>n</i> = 36, DMSO; <i>n</i> = 32, MTZ. ***0.0003 via an unpaired <i>t</i> test. The underlying data for the graphs in this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002194#pbio.3002194.s017" target="_blank">S2 Data</a>.</p> <p>(PDF)</p>