UNC-31 cells autonomously facilitate excitatory evoked release in cholinergic motor neurons.

(<b>A</b>) Representative traces of evoked EPSCs recorded from wild-type, <i>unc-31(e928)</i>, and rescue strains <i>unc-31(e928); gaaEx1302</i>. (<b>B</b>, <b>C</b>) Quantification of the eEPSC amplitude (<b>B</b>) and charge transfer (<b>C</b>) recorded from the strains mentioned in (<b>A</b>). <i>n</i> ≥ 11 animals. Defects in eEPSCs of <i>unc-31</i> mutants can be rescued by expressing UNC-31 cDNA in acetylcholine neurons. The data are presented as box-and-whisker plots, with the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers) indicated. One-way ANOVA was used for comparisons of multiple groups (F_{(2, 35)} = 19.05, <i>P</i> < 0.0001 for <b>B</b>; F_{(2, 35)} = 42.14, <i>P</i> < 0.0001 for <b>C</b>), followed by Tukey’s range test, * <i>P</i> < 0.05; ** <i>P</i> < 0.01; *** <i>P</i> < 0.001. The error bars represent the SEM. <i>N</i> = 3 independent replicates. All the raw data associated with this figure are available in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003023#pbio.3003023.s016" target="_blank">S1 Data</a>. (PDF)