Effects of a canned infant juice auxiliary food on acute inflammation in young mice and antioxidant activity

ObjectiveTo investigate the effects of an infant juice canned auxiliary food on acute inflammation and antioxidant activity in young mice.Methods70 mice were divided into blank group, model group, low dose group (8.697 9 g/kg), middle dose group (17.395 8 g/kg), high dose group (34.791 6 g/kg), high dose group (69.583 2 g/kg) and positive drug control group. Adopting croton oil-induced acute ear edema model in mice to evaluate the effects of juice canned supplementary food on acute inflammation in young mice; calculated the swelling rate of left ear in each group of mice except the blank group. The levels of TNF-α, IL-2, IL-6 and IL-1β in serum were detected by ELISA. HE staining was used to detect the pathological morphology of liver tissue in mice. Electronic tongue for taste analysis. The antioxidant capacity (DPPH free radical scavenging ability, ·OH free radical scavenging ability of infant canned auxiliary food was determined by ultraviolet spectrophotometry.ResultsCompared with the blank group, the left ear swelling of the model group was obvious, the contents of TNF-α, IL-6 and IL-1β in serum were significantly increased (P<0.05), the content of IL-2 was significantly decreased (P<0.05), and the infiltration of inflammatory cells in liver tissue was obvious. Compared with the model group, the swelling degree of the left ear of the mice in the low, medium, high and ultra-high dose groups gradually decreased with the increase of the dose (P<0.05), and the contents of TNF-α, IL-6 and IL-1β in the serum decreased. The difference was statistically significant (P<0.05), and the degree of decrease increased with the increase of the dose. The content of IL-2 in the high-dose and ultra-high-dose groups of mice increased (P<0.05), and the inflammatory symptoms were alleviated. The results of HE staining showed that the degree of nuclear swelling in liver tissue of mice in the medium, high and ultra-high dose groups was significantly improved, and the diffuse inflammatory cell infiltration was reduced. The antioxidant results showed that the IC50 of DPPH free radical scavenging rate and OH free radical scavenging rate were 0.023 mg/mL and 0.022 mg/mL (based on total phenols in the sample), respectively.ConclusionThis kind of infant juice canned auxiliary food has a good anti-inflammatory effect on the acute inflammation of young mice caused by croton oil.