Analysis of proteomic and phosphoproteomic changes during sexual differentiation.

<p>Changes in the proteome and phosphoproteome of mating cells in a time course starting at t0 = cell mixing on MSL-N plates in the dark. The cells were pre-grown separately in liquid MSL-N for 2 h. All Data are corrected for changes happening during starvation in absence of a mating partner. (<b>A</b>) Heatmap of the significant changes in the levels of 54 proteins during mating, showing 2 major clusters of proteins whose level increase (1) or decrease (2). The underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002963#pbio.3002963.s008" target="_blank">S1 Table</a>. (<b>B</b>) Significant fold enrichment in GO annotations for biological processes, molecular functions, and cellular components in proteins whose level increases during mating. No significant enrichment was found for the few proteins whose levels decrease. (<b>C</b>) Significant fold enrichment in GO annotations for biological processes, molecular functions, and cellular components of proteins containing one or several sites showing phosphorylation increase during nitrogen starvation. (<b>D</b>) Significant fold enrichment in GO annotations for biological processes, molecular functions, and cellular components of proteins containing one or several sites showing phosphorylation decrease during nitrogen starvation. Significance levels were assessed by Fisher’s exact test and corrected for false discovery rate. (<b>E</b>) Analysis of the protein sequence surrounding serine and threonine residues phosphorylated during mating. The height of the amino acid one-letter code represents the frequency. The logo on the left was made using all 192 increasing phosphosites. For the one on the left, all proline-directed sites and Orb6 substrates were removed, leaving 122 phosphosites.</p> <p>(PDF)</p>