TAR triggered by TPA-UVB dual treatment in mouse skin is partially PKCα-dependent.

<p><b>(A)</b> Quantitation of total PKCα levels in epidermis of WT and <i>Krt17-/-</i> mouse skin 6 h after dual TPA Tx, 24 h apart. <i>n</i> = 3 mice. Data reported as mean ± SEM. One-way ANOVA. <b>(B)</b> WT and <i>Krt17-/-</i> mouse ears were treated with TPA followed by UVB 24 h later. Tissues were collected 6 h after UVB and immunostained for phosphor-PKCα (T638), K14, and nuclei (DAPI). Scale bars: 50 μm. epi, epidermis; derm, dermis; hf, hair follicle. Dashed lines depict the dermo-epidermal interface. <b>(C)</b> Quantitation of the phospho-PKCα signal in data from (B). <i>n</i> = 4 mice. Data are shown as mean ± SEM. One-way ANOVA. <b>(D)</b> Impact of pretreatment with the PKCα inhibitor Go6976 30 min prior to the 2nd Tx, UVB (24 h interval), in WT mouse ear skin. Sections were immunostained for Ly6g, K17, K14, and nuclei (DAPI). Scale bars: 50 μm. Asterisks denote the loss of epidermis-proximal pool of neutrophils when pretreated with Go6976. <b>(E, F)</b> Quantitation of neutrophil fluorescence signals using surface area measurements (E) and line vectors extending from the basement membrane to the bottom of the dermis (F). <i>n</i> = 3 mice. Data reported as mean ± SEM. One-way ANOVA. The source data used to derive the numerical values reported here can be found in <b><a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002779#pbio.3002779.s016" target="_blank">S1 Data</a></b>.</p> <p>(PDF)</p>