Silencing <i>Vangl2</i> promotes the activation of NLRP3 inflammasome in macrophages.

(A) PEMs were silenced with scramble siRNA or <i>Vangl2</i> siRNA for 24 h, and the expression of VANGL2 was detected by immunoblot analysis. (B) PEMs were transfected with scramble siRNA or <i>Vangl2</i> siRNA for 24 h, followed by the treatment of LPS (100 ng/ml) for 6 h, and then inflammasome-related agonists were added for indicated times. Finally, the expression of IL-1β was detected by ELISA. (C) BMDMs were transfected with scramble siRNA or <i>Vangl2</i> siRNA for 24 h, followed by LPS (100 ng/ml) treatment for 6 h, and then added the inflammasome-related activators for indicated times. Finally, ELISA was used to detect the expression of the IL-1β. (D) PEMs were transfected by scramble siRNA or <i>Vangl2</i> siRNA for 24 h, followed by LPS (100 ng/ml) treatment for 6 h, and then added NLRP3 inflammasome agonists. Finally, the expression of inflammasome-related proteins were detected by immunoblot analysis. (E) Immunoblot analysis was used to detect the expression of p-p65, p65, p-IκBα, and IκBα in the colon of WT and <i>Vangl2</i>^ΔM/ΔM mice with DSS-induced colitis. (F, G) ELISA was used to detect the expression of IL-6 (F) and TNF-α (G) in the colon of WT and <i>Vangl2</i>^ΔM/ΔM mice with DSS-induced colitis. Data are shown as means ± SD. *<i>P</i> < 0.05, **<i>P</i> < 0.01, NS means not significant. (PDF)