Analysis of fungal community structure of medium-high temperature Daqu in Hetao area

The fungal flora diversity and community structure of the medium-high temperature Daqu in Hetao area of Bayannur, Inner Mongolia was analyzed by Illumina MiSeq high-throughput sequencing technology, and compared with the fungal flora of medium-high temperature Daqu in Yingtan area of Jiangxi, and the characteristics of fungal flora were analyzed. The results showed that the fungal phyla in the medium-high temperature Daqu samples in Hetao area were mainly Ascomycota, while the fungal genera were Thermomyces, Thermoascus, Aspergillus, and Pichia. The diversity comparison showed that compared with the Yingtan area, the richness and diversity of fungal flora in the medium-high temperature Daqu samples in Hetao area were significantly lower (P<0.05). By comparing key fungal genera, it was found that the Thermoascus and Thermomyces were more abundant in medium-high temperature Daqu samples in Hetao area, while the relative abundance of Rhizopus, Aspergillus and Lichtheimia was less. By comparing correlation analysis results, it was found that there were significant differences in the correlation between fungi genera in the samples from the two regions, among them, the relationship between fungi genera was closer and more stable in the samples from the middle and high temperature Daqu in Hetao area. In conclusion, Thermomyces, Thermoascus, Aspergillus and Pichia were abundant in medium-high temperature Daqu in Hetao region, and there were some differences in the fungal community structure of medium-high temperature Daqu produced in different regions.