Optimization of fermentation conditions for low-temperature sterol esterase production by Pantoea agglomerans from marine

In order to improve the enzymatic activity of low-temperature sterol esterase produced by Pantoea agglomerans Y-03 from marine, the significant factors affecting enzymatic activity of low temperature sterol esterase produced by P. agglomerans Y-03 were determined by single factor tests and Plackett-Burman (PB) tests, and the fermentation process was optimized by Box-Behnken (BB) response surface tests. The results showed that the optimal fermentation process for producing low-temperature sterol esterase by P. agglomerans Y-03 were glucose 3.7%, tryptone 1.4%, K₂HPO₄ 0.4%, initial pH 8, rotational speed 180 r/min, inoculum 4%, liquid loading volume 45 ml/250 ml, fermentation temperature 27 ℃. Under these optimal conditions, the sterol esterase activity was 484.24 U/L, which was 143.89% higher than that before optimization. This study laid a foundation for improving the yield and activity of sterol esterase produced by P. agglomerans and for future research on scale-up production.