Study on the detection and stability of 8 effective components in compound health wine

A method for the determination of 8 effective components (5-hydroxymethylfurfural, adenosine, cordycepin, N6-(2-hydroxyethyl) adenosine, picrocrocin, rutin, crocin I and crocin II in compound health wine was established by HPLC, and the stability of 8 effective components was analyzed. The results showed that the optimal chromatographic conditions were as follows: Agilent SB-Aq C₁₈ column (4.6 mm×250 mm, 5 μm), acetonitrile- 0.1% acetic acid aqueous as mobile phase, flow rate 1.0 ml/min, detection wavelength 260 nm/440 nm, column temperature 30 ℃, and injection volume 10 μl. Under the analytical conditions, the 8 effective ingredients showed good linear relationships in their respective mass concentration ranges (R²≥0.999 9). The limits of detection (LOD) were 15.5-146.1 μg/L, and the limits of quantitation (LOQ) were 45.5-429.7 μg/L. The relative standard deviations (RSD) of precision, stability and repeatability tests were all lower than 2.0%. The average spiked recovery rates were 96.15%-99.23%. The stability tests results of samples showed that the adenosine, cordycepin, N6-(2-hydroxyethyl) adenosine and picrocrocin were stable under different storage conditions for 90 d, the 5-hydroxymethylfurfural was sensitive to temperature, and the rutin, crocin I, and crocin II were extremely sensitive to light and heat.