Analysis and dietary risk assessment of 12 pesticide residues in wine

335 pesticides in wine were preliminarily screened by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), 12 pesticides with high detection rate, such as propamocarb were selected and determined by QuEChERS-HPLC-MS/MS, and their dietary risk was evaluated. The results showed that each pesticide was extracted by acetonitrile, purified by QuEChERS method, ionized by electrospray ionization positive ions (ESI⁺) mode, determined by multiple reaction monitoring (MRM) mode, and quantitatively analyzed by matrix matching standard solution external standard method. The linear relationships of 12 pesticides were good in the mass concentration range of 1-200 ng/ml, and the correlation coefficients R were all greater than 0.997. The limits of detection (LOD) and limits of quantitation (LOQ) were 0.000 5 mg/kg and 0.001 mg/kg, respectively. The average standard recovery rates were 82%-110%. The relative standard deviation (RSD) of the precision tests was 2.5%-9.2%. The results of dietary risk assessment showed that the chronic risk entropy (RQc) of 11 pesticides were 1.2×10^-6-1.1×10^-3, and the acute risk entropy (RQa) of 5 pesticides were 9.2×10^-6-2.5×10^-3, indicating that the pesticide residues such as propamocarb, acetamiprid, carbendazim, thiophanate methyl and dimethomorph in wine did not pose an unacceptable risk to consumers.