Ways to increase the effectiveness of hyperimmune serum against colibacteriosis, salmonellosis and pasteureellosis of lambs

\nThis article presents materials from scientific research on the development of methods for obtaining antigens for hyperimmunization from pathogens of escherichiosis, salmonellosis and pasteurellosis of lambs and calves with high immunogenicity. Corpuscular, autolysis, hydrolysis, and toxic antigens were obtained. From the obtained antigens, a mixed fraction was formed – corpuscular + autolysis, hydrolysis + toxic, fractions with the addition of aethonium. In experiments on hyperimmunization of rabbits, a relatively high titer (1:25600) was established when using a mixed antigen with the addition of aethonium, compared with the indicators of the other four types of antigens separately. At the same time, the synergism of antigens of three pathogens with simultaneous use has been proven. When tested on rabbits, hyperimmune serum obtained using a mixed antigen with the addition of aethonium showed relatively high therapeutic and preventive effectiveness. When using a mixed antigen with the addition of ethonium, hyperimmunized fattening bulls were subjected to total bleeding with subsequent isolation of blood serum for use in therapeutic and prophylactic purposes. Sera with a titer of specific antibodies greater than 1:10,000 were used. A total of 110 liters of whey were produced. Therapeutic and preventive effectiveness when used in lambs was 95%, and in calves 87%.\n