Screening of Short-Chain\nPeptide RKIIIRW and Its Use\nas a Hydrogel for Wound Healing and Bacterial Infection Treatment

Microbial resistance has gradually emerged as a significant\nchallenge\nin global health care, mainly due to the overuse of antimicrobials.\nIn the face of this challenge, antimicrobial peptides (AMPs) have\nemerged as a potential therapeutic agent due to their excellent antimicrobial\nactivity. In this work, Gram-negative bacteria (E.\ncoli) and Gram-positive bacteria (S.\naureus) were selected as bacterial models. The membrane\nchromatographic stationary phase was prepared by extracting the membranes\nof E. coli and S. aureus and fixing them on the silica microspheres. According to the principle\nof electrostatic adsorption of bacteria by AMPs to kill bacteria,\nwe designed and developed a series of short-chain AMPs. Then, we investigated\nthe cationic AMP RKIIIRW, which effectively killed E. coli and S. aureus, using cell membrane chromatography combined with high-performance\nliquid chromatography. We prepared a kind of antibacterial peptide\nhydrogel with sodium alginate/hyaluronic acid to treat bacterial infections\nand promote wound healing. The prepared antibacterial hydrogel inhibits\nbacterial infection in the damaged skin by slowly releasing the antibacterial\npeptide RKIIIRW while promoting the growth of skin tissue cells and\naccelerating wound healing. In summary, we have successfully screened\nAMPs using membrane chromatography and developed a hydrogel treatment\nsystem that can inhibit bacterial infection and promote skin wound\nhealing.