BioactiveZn–V–Si–Ca Glass NanoparticleHydrogel Microneedles with Antimicrobial and Antioxidant Propertiesfor Bone Regeneration in Diabetic Periodontitis

Periodontitis is a chronic inflammatory condition affecting the periodontal tissue. This condition worsens in diabetic patients due to oxidative stress and inflammation. Herein, we investigated a treatment using bioactive Zn–V–Si–Ca glass nanoparticle hydrogel microneedles. The microneedles contain bioactive glass nanoparticles codoped with zinc and vanadium ions. They also include gallic acid and oxidized methacrylated hyaluronic acid. These microneedles address bacterial dysbiosis and oxidative stress in diabetic periodontitis. They provide antibacterial and antioxidant effects. The microneedles deliver therapeutic agents directly into the gingival tissue. This enhances drug retention and absorption by penetrating the mucosal barrier. <i>In vitro</i> studies demonstrated biocompatibility, excellent antioxidant properties, and acceptable mechanical properties. Meanwhile, the microneedle patches demonstrated antibacterial properties effective against a Gram-negative periodontal pathogen as well as a Gram-positive oral bacterium. <i>In vivo</i> experiments were performed using a diabetic rat model with periodontitis. Results showed significant improvement in alveolar bone regeneration. The hydrogel modulated the inflammatory microenvironment effectively. Ribonucleic acid sequencing revealed downregulation of JAK-STAT and NF-κB inflammation signaling pathways. This work presents a distinctive approach to suppressing the inflammatory response and modulate immune responses for the purpose of treating diabetic periodontitis early.