Synergistic\nCo-Assembly of Natural Oxidase and Peptide-Heme\nNanozyme for In Situ H<sub>2</sub>O<sub>2</sub> Removal with Enhanced\nCatalytic Efficiency and Stability

Hydrogen peroxide, a byproduct of the oxidase catalytic\nprocess,\nsignificantly compromises the enzymatic activity and stability of\nnatural oxidases, thereby restricting their industrial applications.\nTo address this challenge, we developed a coassembly system combining\na natural oxidase with a peptide-heme (PepHeme) nanozyme possessing\ncatalase-like activity on a ferritin platform. This system, demonstrated\nby using natural choline oxidase and galactose oxidase as models,\nforms an oxidase-catalase cascade reaction. Leveraging the self-assembly\nproperties of ferritin and the directional association enabled by\nsortase ligation, we successfully constructed clusters of oxidases\nand PepHeme nanozymes. This design facilitated the directional immobilization\nof natural enzymes and nanozymes and increased the local concentration\nof these cascade clusters, thus significantly enhancing both the catalytic\nefficiency and stability of the enzyme, especially the hydrogen peroxide\ntolerance and long-term stability. This cascade system offers a promising\nsolution to the hydrogen peroxide byproduct issue in the industrial\napplications of oxidases.