Mesenchymal\nStem Cells Engineered by Multicomponent\nCoassembled DNA Nanofibers for Enhanced Wound Healing

A major challenge for stem cell therapies, such as using\nmesenchymal\nstem cells to treat skin injuries, is the stable engraftment of exogenous\ncells and the maintenance of their regenerative capacities in the\nwound areas. DNA-based self-assembly strategies can be used for artificial\nand multifunctional cell surface engineering to stabilize and enhance\ntheir functions for therapeutic applications. Here, we developed DNA\nnanofiber-decorated stem cells, in which DNA-based, multivalent fiber-like\nstructures were self-assembled in situ on the cell surfaces. These\nengineered stem cells have demonstrated robust reactive oxygen species\n(ROS) scavenging effects, specific adhesion to damaged vascular endothelial\ncells, and the ability to enhance angiogenesis, which were effective\nand safe for acute or chronic wound healing in a mouse model with\nexcisional skin injury. This DNA nanostructure-engineered stem cell\nprovides a novel therapeutic platform for the treatment of tissue\ndamage.