3D Printed\nCoated Blade Spray–Mass Spectrometry\nDevices

Coated blade spray–mass spectrometry (CBS-MS)\nhas emerged\nas a powerful tool for the rapid screening of target compounds at\ntrace levels in complex biological matrices. Despite its potential,\nthe broader adoption of CBS-MS technology has been hindered by the\nlack of commercially available, user-friendly MS interfaces and extraction\ndevices. In this work, we present comprehensive CBS-MS solutions developed\nusing 3D printing, including a versatile MS interface and two extraction\ndevices tailored to different analytical needs: one is compatible\nwith LC vials for large-volume samples, while the other is optimized\nfor single-drop blood analysis. The MS interface features a novel\ndesign that separates the immobilization station from the blade holder,\nsignificantly simplifying the operational workflow and minimizing\nthe contamination risks and hazards associated with manual blade handling.\nThe first extraction cartridge can process 48 samples simultaneously\nwith an average sample preparation time of less than 20 s, while the\nsecond extraction device enables extraction from 8 single-drop blood\nsamples via on-blade extraction. The developed devices were successfully\ntested for the rapid screening of seven drugs in both urine and single-drop\nblood samples, demonstrating a promising analytical performance. Additionally,\npotential contamination issues related to the use of 3D-printed materials\nas extraction phases were examined, emphasizing the importance of\nensuring that 3D-printed materials do not leach contaminants into\nsamples or solvents and contaminate the MS.