Visualizing\nDynamic Weak Interaction Networks of Fluorine\nAtoms within Proteins via NMR

The utilization of fluorine probes in protein research\nhas advanced\nour understanding of the nature of macromolecules. The diverse activities\nof proteins are governed by intricate weak interaction networks, yet\nthe experimental detection presents a challenge. Herein, we have developed\nan NMR analytical method based on quantum coherent operations to characterize\nthe weak chemical bonds of fluorine atoms within proteins that are\nbound to metal fluorides or labeled with fluorinated amino acids.\nOur approach offers a fast-screening method for identifying a weak\ninteraction network without requiring crystallization.