DNA Origami\nIncorporated into Solid-State Nanopores\nEnables Enhanced Sensitivity for Precise Analysis of Protein Translocations

The rapidly advancing field of nanotechnology is driving\nthe development\nof precise sensing methods at the nanoscale, with solid-state nanopores\nemerging as promising tools for biomolecular sensing. This study investigates\nthe increased sensitivity of solid-state nanopores achieved by integrating\nDNA origami structures, leading to the improved analysis of protein\ntranslocations. Using holo human serum transferrin (holo-hSTf) as\na model protein, we compared hybrid nanopores incorporating DNA origami\nwith open solid-state nanopores. Results show a significant enhancement\nin holo-hSTf detection sensitivity with DNA origami integration, suggesting\na unique role of DNA interactions beyond confinement. This approach\nholds potential for ultrasensitive protein detection in biosensing\napplications, offering advancements in biomedical research and diagnostic\ntool development for diseases with low-abundance protein biomarkers.\nFurther exploration of origami designs and nanopore configurations\npromises even greater sensitivity and versatility in the detection\nof a wider range of proteins, paving the way for advanced biosensing\ntechnologies.