Synergetic\nEnzyme-Incorporated Metal–Organic\nFramework and Polyoxometalate Nanozyme: Achieving Stable Tandem Catalysis\nfor Organic Photoelectrochemical Transistor Bioanalysis

Organic\nphotoelectrochemical transistor (OPECT) has emerged\nas\na promising technique for biomolecule detection, yet its operational\nrationale remains limited due to its short development time. This\nstudy introduces a stable tandem catalysis protocol by synergizing\nthe enzyme-incorporated metal–organic frameworks (E-MOFs) with\npolyoxometalate (POM) nanozyme for sensitive OPECT bioanalysis. The\nzeolitic imidazolate framework-8 (ZIF-8) acts as the skeleton to protect\nthe encapsulated glucose oxidase (GOx), allowing the stable catalytic\ngeneration of H<sub>2</sub>O<sub>2</sub>. With peroxidase-like activity,\na phosphotungstic acid hydrate (PW<sub>12</sub>) is then able to utilize\nthe H<sub>2</sub>O<sub>2</sub> to induce the biomimetic precipitation\non the photogate, ultimately resulting in the altered device characteristics\nfor quantitative detection. This work reveals the potential and versatility\nof an engineered enzymatic system as a key enabler to achieve novel\nOPECT bioanalysis, which is believed to offer a feasible framework\nto explore new operational rationale in optoelectronic and bioelectronic\ndetection.