Diazotisation Coupling Reaction for Spectrophotometric Colorimetric Identification of Paracetamol in Pharmaceutical Samples

This study presents a new straightforward, and precise spectrophotometric technique for determining the amount of paracetamol. The azo dye is formed using a new chromogenic reagent orcinol to produce an orange-reddish result that is detected at 514 nm. UV-visible and other spectroscopy methods were used to confirm the newly synthesized compound's molecular structure. A recently produced chemical was tested against a number of bacterial species in vitro. A linear connection between the concentration range of 0.8-20.5 µg. mL-1 of paracetamol was achieved under carefully controlled experimental conditions that impact the response. In addition to the molar absorptivity of 12.54×106 L.mol-1.cm-1, the regression coefficient (R2) was 0.9961. For the purpose of determining paracetamol in the pharmaceutical business, the suggested methodology was successfully adjusted. Paracetamol, also known as N-(4-hydroxy-phenyl) acetamide, is a common medication used to treat headaches and other mild aches and pains. It can also be used to treat more severe pains while limiting adverse effects by allowing the use of other non-steroidal anti-inflammatory medicines at lower dosages