Carbapenem Resistance Associated with Biofilm Formation in Clinical Pseudomonas aeruginosa Isolates

Background: Pseudomonas aeruginosa intrinsic and acquired resistance to specific antibiotics contributes to the increased prevalence of infections acquired in hospitals. Objective: This study aims to identify the presence of key biofilm-associated genes (algD and pslD) and carbapenemase-encoding genes (VIM and NDM) among local carbapenem-resistant P. aeruginosa (CRPA) isolates. Methodology: The existence of the genes algD, pslD, VIM, and NDM-1 was examined in 30 CRPA isolates. Chi-square analysis was used to evaluate the connection between carbapenem resistance and biofilm density. Results: Among 30 isolates tested, algD and NDM-1 genes were detected most frequently (93.3%), while pslD and VIM genes were found at lower rates (86.6% and 78.5%, respectively). “Carbapenem resistance and Pseudomonas aeruginosa ability to form biofilm were shown to be significantly correlated.” Chi-square analysis revealed a strong relationship between biofilm density and carbapenem resistance (X² = 29.007, p ≤ 0.01), Conclusion: Biofilm development in Pseudomonas aeruginosa contributes to infection persistence and. resistance mechanisms and highlighting the critical role of biofilm in enhancing resistance mechanisms and infection persistence.