Evaluation of the influence of RNA extraction methods on the detection of citrus viruses and viroids

Despite the differential pathogen extraction amongst protocols, all target pathogens could be detected with appropriate RT-qPCR replicates and combined bioinformatic approaches. This study emphasises that although multiple protocols can be effective, further selection and optimisation could enhance the performance. Present research serves as a foundation for the refinement of diagnostic pipelines in citrus pathology.