Identification for GmCDPK3 Gene Family Effect on Symbiosis Based on the Genetic Mapping in Soybean (Glycine max)

Nitrogen is an essential element for normal growth and development of plants.Scientific research evidence on biological nitrogen fixation (BNF) can not only effectively solve the problem of low nitrogen utilization rate and pollution, and conducive to the development of friendly agriculture.Although BNF plays an important role in economic and environmental, there have been few reports on quantitative trait loci (QTL) controlling BNF traits in soybeans.This study focuses on identify QTLs for the total nodule number (TNN) in the soybean population field of 'Suinong 14' 'ZYD00006' (SZ, n=160) chromosome segment substitution line (CSSLs).Field collected samples were used to collect phenotypic data of TNN.QTL underlying TNN was analyzed, four QTLs were identified in CSSLs and these QTLs were located on (LGs) D1b, A1, A2 and K, respectively.Gene annotation on these QTLs, indicated that genes encoding proteins were related to signaling transduction.Among them, the gene Glyma.05g213200which located on chromosome 5 belongs to Ca 2+ dependent protein kinases3 (CDPK3) family, which regulated Ca 2+ signaling transduction.We also analysis another two genes belong to CDPK3 family.Making systematic analysis of this family, to lay the foundation for exploring the relationship between CDPK and nodulation.And the qRT-PCR result support that CPK-1(Glyma.05g213200)and CPK-3 (Glyma.01g223200)are the two main genes induced by rhizobium.These results provide important candidate genes for exploring the symbiotic relationship between soybean and rhizobium.CPK-1 plays a more important role in inducing nodulation, and CPK-1 could be used for further research.