Optimization of Ultrasound-Assisted Extraction of Pigment from Tremella sanguinea

Tremella sanguinea Peng, one of the species in Tremella family, was an edible and medicinal fungus.Its fruit bodies were rich in natural yellow pigment with some nice features, such as thermo-stability, acid stability and water solubility.Thus, it had a wide prospect in business application; but till now, the standardized extraction process combining with ultrasound-assisted extraction (USAE) technique and the optimal parameters had not been reported.In our research, the pigment was isolated and analyzed by high performance liquid chromatography (HPLC).Plackett-Burman (PB) design, steepest ascent experiments and response surface methodology (RSM) design were applied to screen out the optimal parameter values of extraction with ultrasound assistance.The results showed that the maximum absorption wavelength of the pigment was 450 nm.Ultrasound power, extract temperature, solvent concentration and solvent sample ratio had significant influence on extraction values.When the input power, extract temperature, ethyl alcohol concentration and solvent sample ratio were 248.01 W, 87.37C, 44.04% and 152.78, respectively, the pigment yield reached a maximum of 885.55 U/g.These findings contributed to the establishment of USAE procedure for T. sanguinea Peng pigment.