Margarine Consumption Induces Oxidative Stress in the Gut of Wistar Albino Rats

Diet has a great impact on human health and consumption of high caloric diets (rich in fat) may lead to metabolic disorders.High fat diets can trigger dysbiosis, cellular stress, intestinal epithelial permeability, onset of inflammation and ultimately CRC.The current research was designed to evaluate the effect of HFD on gut pathophysiology.For this purpose, HFD (15% and 30% Margarine: Blue Band ) was fed to Wistar rats for a period of 6 weeks (42 days).Blood serum samples were collected for evaluating liver, renal function markers, serum, Total Oxidative Stress (TOS) and Total Antioxidant Capacity (TAC).Tissue sampling from the gut (Ileum) of experimental animals was done to perform histopathology and cellular ROS (Reactive Oxygen Species) detection.The data were statistically analyzed by applying two-way ANOVA and DMR (Duncan's Multiple Range).Results revealed significant (P 0.05) role of HFD in elevating body TOS (10.151.47 and 10.201.30) in comparison to the control group (3.320.72).The onset of inflammation was observed in response to HFD feeding even after a short time period (10 th day) in the intestinal epithelium.Apparently, the induction was triggered by HFD mediated stress response.Significantly higher (P0.05)cellular stress (cellular ROS production), was also observed in HFD fed groups compared to the control group.Taken together, HFD has a major impact on different aspects of Wistar rat intestinal physiology and it induces oxidative stress due to onset of cellular stress pathways.