Integrating real-time OUR monitoring with adaptive feeding for enhanced antibody production
The RF strategy resulted in a mismatch between nutrient supply and cellular demand during LSP, triggering osmotic stress and limiting antibody expression. In contrast, the OBCF strategy dynamically aligned nutrient delivery with cellular respiration, thereby mitigating osmotic stress and reshaping intracellular metabolism. Notably, OBCF enhanced pyruvate utilization and TCA cycle activity, promoted amino acid catabolism, and suppressed by-product accumulation. These metabolic improvements led to a 52% increase in specific productivity (Q<sub>P</sub>) and a 32% increase in total antibody yield during LSP, along with reduced batch-to-batch variability.
Authors
- Wen‐Song Tan (ORCID: https://orcid.org/0000-0001-9857-4798)
- Yongmei He (ORCID: https://orcid.org/0000-0001-8072-7514)
- Liang Zhao (ORCID: https://orcid.org/0000-0002-3361-0783)
- Botao Zhang (ORCID: https://orcid.org/0000-0003-3691-7876)
- Xinran Zhang (ORCID: https://orcid.org/0000-0001-7433-7974)
- Qian Ye
- Qingyuan Ran
Institutions
- East China University of Science and Technology (CN)
- University of Shanghai for Science and Technology (CN)
- Shanghai Genon Biological Products (China) (CN)
- Qingdao Institute of Bioenergy and Bioprocess Technology (CN)
Publication Details
- Journal
- Bioresources and Bioprocessing
- Published
- 2025-12-20
- DOI
- https://doi.org/10.1186/s40643-025-00961-x
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.00