Identification of anti-inflammatory constituents of <i>lindera glauca</i> using cell extraction coupled with lC-QTOF MS/MS

<i>Lindera glauca</i>, an edible medicinal plant native to Northeastern Asia, has been reported to possess various pharmacological activities. This study employed cell extraction (CE) in conjunction with LC-QTOF-MS/MS to identify bioactive components that bind to target cells. The 80% MeOH extract of <i>L. glauca</i> exhibited significant nitric oxide (NO) production inhibitory activity in BV2 cells and was applied to the CE coupled with the LC-QTOF MS/MS method. New compound 3,5-dimethoxyphenyl-4-hydroxymethyl-β-ᴅ-glucopyranoside (<b>2</b>), and five known compounds (<b>1</b>, <b>3</b>-<b>6</b>) were isolated from <i>L. glauca.</i> All compounds displayed anti-inflammatory activities, and compounds <b>2</b>-<b>3</b> inhibited NO production in BV2 cells. In molecular docking simulations, compound <b>2</b> showed high binding affinities to inflammatory marker proteins COX-2 and iNOS. In conclusion, the CE method combined with LC-QTOF MS/MS effectively facilitates the rapid identification of potential active compounds. And new compound <b>2</b> from <i>L. glauca</i> may be a new candidate against inflammatory diseases.