Fabrication of coumarin-loaded zeolitic imidazole nanocomposite for antimicrobial and wound healing applications an in vitro assessment

A multifunctional coumarin (CU) encapsulated zeolitic imidazole frameworks nanocomposite (CU@ZIF-90) was fabricated for efficient wound healing application. The stimuli-responsive drug release mechanism was examined at pH 5 and 7.4. The release of coumarin was increased within three hours by burst release under acidic conditions. The CU@ZIF-90 shows significant bacterial reduction against human pathogens S. aureus, E. coli, P. aeruginosa, and S. epidermidis. Moreover, the MTT cytotoxicity assays revealed that the nanocomposite had high cell viability. The study primarily focuses on the fabrication of CU@ZIF-90 nanocomposite for stimuli-responsive targeted drug delivery. The synthesized CU@ZIF-90 nanocomposite demonstrates a high level of thermal stability. The anti-biofilm results confirmed that the nanocomposite effectively inhibits biofilm formation and damages the exopolysaccharide matrix in a dose-dependent manner. Wound scratch assay revealed that the controlled release of coumarin and zinc promotes the cell proliferation and migration mechanisms. Bio-compatibility study indicates that the CU@ZIF-90 nanocomposite is a non-toxic material for external clinical uses. The overall results demonstrated that the CU@ZIF-90 nanocomposite is an efficient and low-cost material for antimicrobial and wound healing applications.