Culture conditions impact the protein expression profile of Mycobacterium bovis BCG strains Moreau and Pasteur

The analysis of the intracellular 2DE profile reveals differences between cultivation methods as well as between the two M. bovis BCG vaccine strains, Moreau and Pasteur. Our results identify important proteins that may contribute to elucidating differences in vaccine efficacy, highlighting the importance of culture conditions when comparing different studies. These findings contribute to the characterization of the Brazilian vaccine strain, BCG Moreau under growth conditions similar to those used in vaccine production.