A pilot study on a heterologous prime-boost approach for inducing an immune response against double-layered human rotavirus particles

This study indicated that immune stimulation can vary based on the combination of antigens and the order in which they are introduced into the host. According to our findings, a heterologous regimen composed of recombinant VP6 protein with complete Freund's adjuvant as the priming shot and rotavirus particles with incomplete Freund's adjuvant as the booster shot could elicit an antibody response at least two-fold stronger than the other homologous regimens applied in the present study. This preliminary observation suggests a high-yielding yet cost-effective protocol to access abundant anti-VP6 polyclonal antibodies as a base for different DLP-relying research.