Thermorhabdus hydrogeniformans gen. nov., sp. nov., a thermophilic anaerobic bacterium from hot spring sediments in Tengchong, Yunnan, China
A novel member of the phylum <i>Bacillota</i> was isolated from Tengchong Hot Spring sediments (Yunnan, China). Cells are Gram-stain-negative, anaerobic, rod-shaped bacteria (0.3-0.5 µm wide and 1.5-3.5 µm long) and flagellated. Optimal growth occurs anaerobically at 60 °C (range 50-70 °C), pH 7.0 (range 6.0-8.0) and 0 g l<sup>-1</sup> NaCl (range 0-50 g l<sup>-1</sup>). Substrate utilization encompassed cellobiose, sucrose, soluble starch, casein, glucose, xylan, ethanol, ribose, yeast extract, mannose and tryptone. Comparative 16S rRNA gene-sequence analysis revealed 93.6% similarity to <i>Biomaibacter acetigenes</i> SK-G1<sup>T</sup>. The genome is 2.86 Mbp with a DNA G+C content of 46.5 mol%. Phylogenetic analyses based on the 16S rRNA gene and the whole genome placed strain DL9XJH-110<sup>T</sup> within the family <i>Tepidanaerobacteraceae</i>. The predominant cellular fatty acids (>10%) were C<sub>15 : 0</sub>, iso-C<sub>15 : 0</sub>, C<sub>16 : 0</sub> and C<sub>14 : 0</sub>. Phenotypic, phylogenetic and chemotaxonomic properties indicate that strain DL9XJH-110<sup>T</sup> represents a novel species in a new genus, for which the name <i>Thermorhabdus hydrogeniformans</i> gen. nov., sp. nov. is proposed. The type strain is DL9XJH-110<sup>T</sup> (=CCAM 2419<sup>T</sup>=JCM 39573<sup>T</sup>).
Authors
- Jiahui Xu (ORCID: https://orcid.org/0000-0003-2780-2217)
- Fengping Wang (ORCID: https://orcid.org/0000-0002-3429-8410)
- Tiantian Yu (ORCID: https://orcid.org/0000-0002-2689-3914)
Institutions
- Shanghai Ocean University (CN)
Publication Details
- Journal
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Published
- 2025-12-17
- DOI
- https://doi.org/10.1099/ijsem.0.006997
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00