Plasma Protein Panel for Assessing the Risk of Alzheimer’s Disease by MRM-MS Analysis: The Study of Two Independent Clinical Cohorts
Early recognition of a risk of Alzheimer's disease (AD) remains a global challenge, and blood proteomic markers are of particular interest for wide-scale diagnostic use. Quantitative multiple reaction monitoring (MRM) approach demonstrates good reproducibility in the characteristic changes in the levels of reported candidate biomarkers (CBs) in different cohorts in AD. Following up on our previous study, we performed a joint analysis of 331 blood plasma samples from two different clinical cohorts of participants, comprising a total of 95 samples from patients with AD, 136 samples from patients with mild cognitive impairment (MCI), and 100 samples from controls. The obtained results confirm the significance of 37 CBs. A logistic regression-based algorithm was used to build protein classifiers, and a total of 21 important proteins were selected, 13 of which (ORM1, APOA4, LBP, HP, FN1, BCHE, APOE, PZP, A1BG, TF, SERPINA7, TTR, and F12) formed a universal panel that demonstrated strong classification performance in distinguishing AD patients from controls (ROC-AUC = 0.90) and in separating stable and progressing patients with MCI (ROC-AUC = 0.81). Overall, the analysis confirms the high potential of the MRM method for validating CBs in independent cohorts.
Authors
- Аnna Morozova (ORCID: https://orcid.org/0000-0002-8681-5299)
- Vladimir A. Mitkevich (ORCID: https://orcid.org/0000-0002-1517-1983)
- Alexander Makarov (ORCID: https://orcid.org/0000-0002-7046-6709)
- Polina A. Strelnikova (ORCID: https://orcid.org/0000-0001-6513-8009)
- Natalia V. Zakharova (ORCID: https://orcid.org/0000-0002-5267-0079)
- Maria I. Indeykina (ORCID: https://orcid.org/0000-0002-8177-7449)
- А. С. Кононихин (ORCID: https://orcid.org/0000-0002-2238-3458)
- Dmitry Gryadunov (ORCID: https://orcid.org/0000-0003-3183-318X)
- Marina Emelyanova (ORCID: https://orcid.org/0000-0003-3681-4308)
- Е. Н. Николаев (ORCID: https://orcid.org/0000-0001-6209-2068)
- Elena Fedoseeva (ORCID: https://orcid.org/0000-0003-1134-1401)
- И. В. Колыхалов (ORCID: https://orcid.org/0000-0002-2358-1579)
- Gavrilova Si (ORCID: https://orcid.org/0000-0001-6683-0240)
- Ya. B. Fedorova (ORCID: https://orcid.org/0000-0002-3150-6229)
- Alisa Andryushchenko (ORCID: https://orcid.org/0000-0002-7702-6343)
- Anna E. Bugrova (ORCID: https://orcid.org/0000-0003-1515-9447)
- Yulia A. Chaika
Institutions
- Engelhardt Institute of Molecular Biology (RU)
- Sechenov University (RU)
- Lomonosov Moscow State University (RU)
- Russian Medical Academy of Continuous Professional Education (RU)
- Institute of Biochemical Physics NM Emanuel (RU)
- Mental Health Research Center of Russian Academy of Medical Sciences (RU)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2025-12-19
- DOI
- https://doi.org/10.3390/ijms27010015
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Skolkovo Institute of Science and Technology